pY011 (pcDNA3.1-hLb2Cpf1)

LOCUS       pY011 (pcDNA3.1-hLb2Cpf1) Sequencing Result	9164 bp 	DNA	SYN
DEFINITION  pY011 (pcDNA3.1-hLb2Cpf1) Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="pY011 (pcDNA3.1-hLb2Cpf1) Sequencing Result"
                     /mol_type="other DNA"
     promoter        15..631
                     /label="CMV_immearly_promoter"
     misc_feature    94..381
                     /label="CAG_enhancer"
     misc_feature    548..568
                     /label="CMV_fwd_primer"
     promoter        642..660
                     /label="T7_promoter"
     CDS             708..4463
                     /label="ORF frame 3"
     misc_feature    4380..4406
                     /label="HA"
     misc_feature    complement(4534..4551)
                     /label="BGH_rev_primer"
     terminator      4537..4764
                     /label="bGH_PA_terminator"
     rep_origin      4827..5133
                     /label="f1_origin"
     misc_feature    complement(5247..5267)
                     /label="pBABE_3_primer"
     misc_feature    complement(5253..5468)
                     /label="SV40_enhancer"
     promoter        5265..5533
                     /label="SV40_promoter"
     rep_origin      5432..5509
                     /label="SV40_origin"
     misc_feature    5494..5513
                     /label="SV40pro_F_primer"
     CDS             5648..6442
                     /label="ORF frame 2"
     gene            5651..6439
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(5957..6493)
                     /label="ORF frame 2"
     terminator      6619..6738
                     /label="SV40_PA_terminator"
     misc_feature    6707..6726
                     /label="EBV_rev_primer"
     promoter        complement(6782..6800)
                     /label="M13_reverse_primer"
     misc_feature    complement(6799..6821)
                     /label="M13_pUC_rev_primer"
     promoter        complement(6835..6864)
                     /label="lac_promoter"
     gene            complement(7947..8807)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(7947..8807)
                     /label="ORF frame 1"
     promoter        complement(8849..8877)
                     /label="AmpR_promoter"
ORIGIN
    1 AGATATACGC GTTGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA
   61 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT
  121 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA
  181 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC
  241 TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT
  301 AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG
  361 TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT
  421 GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT
  481 GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC
  541 CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCTC
  601 TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC TCACTATAGG
  661 GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGGTAC CGCCACCATG TACTATGAGT
  721 CCCTGACCAA GCAGTACCCC GTGTCTAAGA CAATCCGGAA TGAGCTGATC CCTATCGGCA
  781 AGACACTGGA TAACATCCGC CAGAACAATA TCCTGGAGAG CGACGTGAAG CGGAAGCAGA
  841 ACTACGAGCA CGTGAAGGGC ATCCTGGATG AGTATCACAA GCAGCTGATC AACGAGGCCC
  901 TGGACAATTG CACCCTGCCA TCCCTGAAGA TCGCCGCCGA GATCTACCTG AAGAATCAGA
  961 AGGAGGTGTC TGACAGAGAG GATTTCAACA AGACACAGGA CCTGCTGAGG AAGGAGGTGG
 1021 TGGAGAAGCT GAAGGCCCAC GAGAACTTTA CCAAGATCGG CAAGAAGGAC ATCCTGGATC
 1081 TGCTGGAGAA GCTGCCTTCC ATCTCTGAGG ACGATTACAA TGCCCTGGAG AGCTTCCGCA
 1141 ACTTTTACAC CTATTTCACA TCCTACAACA AGGTGCGGGA GAATCTGTAT TCTGATAAGG
 1201 AGAAGAGCTC CACAGTGGCC TACAGACTGA TCAACGAGAA TTTCCCAAAG TTTCTGGACA
 1261 ATGTGAAGAG CTATAGGTTT GTGAAAACCG CAGGCATCCT GGCAGATGGC CTGGGAGAGG
 1321 AGGAGCAGGA CTCCCTGTTC ATCGTGGAGA CATTCAACAA GACCCTGACA CAGGACGGCA
 1381 TCGATACCTA CAATTCTCAA GTGGGCAAGA TCAACTCTAG CATCAATCTG TATAACCAGA
 1441 AGAATCAGAA GGCCAATGGC TTCAGAAAGA TCCCCAAGAT GAAGATGCTG TATAAGCAGA
 1501 TCCTGTCCGA TAGGGAGGAG TCTTTCATCG ACGAGTTTCA GAGCGATGAG GTGCTGATCG
 1561 ACAACGTGGA GTCTTATGGC AGCGTGCTGA TCGAGTCTCT GAAGTCCTCT AAGGTGAGCG
 1621 CCTTCTTTGA TGCCCTGAGA GAGTCTAAGG GCAAGAACGT GTACGTGAAG AATGACCTGG
 1681 CCAAGACAGC CATGAGCAAC ATCGTGTTCG AGAATTGGAG GACCTTTGAC GATCTGCTGA
 1741 ACCAGGAGTA CGACCTGGCC AACGAGAACA AGAAGAAGGA CGATAAGTAT TTCGAGAAGC
 1801 GCCAGAAGGA GCTGAAGAAG AATAAGAGCT ACTCCCTGGA GCACCTGTGC AACCTGTCCG
 1861 AGGATTCTTG TAACCTGATC GAGAATTATA TCCACCAGAT CTCCGACGAT ATCGAGAATA
 1921 TCATCATCAA CAATGAGACA TTCCTGCGCA TCGTGATCAA TGAGCACGAC AGGTCCCGCA
 1981 AGCTGGCCAA GAACCGGAAG GCCGTGAAGG CCATCAAGGA CTTTCTGGAT TCTATCAAGG
 2041 TGCTGGAGCG GGAGCTGAAG CTGATCAACA GCTCCGGCCA GGAGCTGGAG AAGGATCTGA
 2101 TCGTGTACTC TGCCCACGAG GAGCTGCTGG TGGAGCTGAA GCAGGTGGAC AGCCTGTATA
 2161 ACATGACCAG AAATTATCTG ACAAAGAAGC CTTTCTCTAC CGAGAAGGTG AAGCTGAACT
 2221 TTAATCGCAG CACACTGCTG AACGGCTGGG ATCGGAATAA GGAGACAGAC AACCTGGGCG
 2281 TGCTGCTGCT GAAGGACGGC AAGTACTATC TGGGCATCAT GAACACAAGC GCCAATAAGG
 2341 CCTTCGTGAA TCCCCCTGTG GCCAAGACCG AGAAGGTGTT TAAGAAGGTG GATTACAAGC
 2401 TGCTGCCAGT GCCCAACCAG ATGCTGCCAA AGGTGTTCTT TGCCAAGAGC AATATCGACT
 2461 TCTATAACCC CTCTAGCGAG ATCTACTCCA ATTATAAGAA GGGCACCCAC AAGAAGGGCA
 2521 ATATGTTTTC CCTGGAGGAT TGTCACAACC TGATCGACTT CTTTAAGGAG TCTATCAGCA
 2581 AGCACGAGGA CTGGAGCAAG TTCGGCTTTA AGTTCAGCGA TACAGCCTCC TACAACGACA
 2641 TCTCCGAGTT CTATCGCGAG GTGGAGAAGC AGGGCTACAA GCTGACCTAT ACAGACATCG
 2701 ATGAGACATA CATCAATGAT CTGATCGAGC GGAACGAGCT GTACCTGTTC CAGATCTATA
 2761 ATAAGGACTT TAGCATGTAC TCCAAGGGCA AGCTGAACCT GCACACACTG TATTTCATGA
 2821 TGCTGTTTGA TCAGCGCAAT ATCGACGACG TGGTGTATAA GCTGAACGGA GAGGCAGAGG
 2881 TGTTCTATAG GCCAGCCTCC ATCTCTGAGG ACGAGCTGAT CATCCACAAG GCCGGCGAGG
 2941 AGATCAAGAA CAAGAATCCT AACCGGGCCA GAACCAAGGA GACAAGCACC TTCAGCTACG
 3001 ACATCGTGAA GGATAAGCGG TATAGCAAGG ATAAGTTTAC CCTGCACATC CCCATCACAA
 3061 TGAACTTCGG CGTGGATGAG GTGAAGCGGT TCAACGACGC CGTGAACAGC GCCATCCGGA
 3121 TCGATGAGAA TGTGAACGTG ATCGGCATCG ACCGGGGCGA GAGAAATCTG CTGTACGTGG
 3181 TGGTCATCGA CTCTAAGGGC AACATCCTGG AGCAGATCTC CCTGAACTCT ATCATCAATA
 3241 AGGAGTACGA CATCGAGACA GATTATCACG CACTGCTGGA TGAGAGGGAG GGCGGCAGAG
 3301 ATAAGGCCCG GAAGGACTGG AACACCGTGG AGAATATCAG GGACCTGAAG GCCGGCTACC
 3361 TGAGCCAGGT GGTGAACGTG GTGGCCAAGC TGGTGCTGAA GTATAATGCC ATCATCTGCC
 3421 TGGAGGACCT GAACTTTGGC TTCAAGAGGG GCCGCCAGAA GGTGGAGAAG CAGGTGTACC
 3481 AGAAGTTCGA GAAGATGCTG ATCGATAAGC TGAATTACCT GGTCATCGAC AAGAGCCGCG
 3541 AGCAGACATC CCCTAAGGAG CTGGGAGGCG CCCTGAACGC ACTGCAGCTG ACCTCTAAGT
 3601 TCAAGAGCTT TAAGGAGCTG GGCAAGCAGT CCGGCGTGAT CTACTATGTG CCTGCCTACC
 3661 TGACCTCTAA GATCGATCCA ACCACAGGCT TCGCCAATCT GTTTTATATG AAGTGTGAGA
 3721 ACGTGGAGAA GTCCAAGAGA TTCTTTGACG GCTTTGATTT CATCAGGTTC AACGCCCTGG
 3781 AGAACGTGTT CGAGTTCGGC TTTGACTACC GGAGCTTCAC CCAGAGGGCC TGCGGCATCA
 3841 ATTCCAAGTG GACCGTGTGC ACCAACGGCG AGCGCATCAT CAAGTATCGG AATCCAGATA
 3901 AGAACAATAT GTTCGACGAG AAGGTGGTGG TGGTGACCGA TGAGATGAAG AACCTGTTTG
 3961 AGCAGTACAA GATCCCCTAT GAGGATGGCA GAAATGTGAA GGACATGATC ATCAGCAACG
 4021 AGGAGGCCGA GTTCTACCGG AGACTGTATA GGCTGCTGCA GCAGACCCTG CAGATGAGAA
 4081 ACAGCACCTC CGACGGCACA AGGGATTACA TCATCTCCCC TGTGAAGAAT AAGAGAGAGG
 4141 CCTACTTCAA CAGCGAGCTG TCCGACGGCT CTGTGCCAAA GGACGCCGAT GCCAACGGCG
 4201 CCTACAATAT CGCCAGAAAG GGCCTGTGGG TGCTGGAGCA GATCAGGCAG AAGAGCGAGG
 4261 GCGAGAAGAT CAATCTGGCC ATGACCAACG CCGAGTGGCT GGAGTATGCC CAGACACACC
 4321 TGCTGAAAAG GCCGGCGGCC ACGAAAAAGG CCGGCCAGGC AAAAAAGAAA AAGGGATCCT
 4381 ACCCATACGA TGTTCCAGAT TACGCTTATC CCTACGACGT GCCTGATTAT GCATACCCAT
 4441 ATGATGTCCC CGACTATGCC TAAGAATTCT GCAGATATCC AGCACAGTGG CGGCCGCTCG
 4501 AGTCTAGAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC
 4561 CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG
 4621 TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC
 4681 TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG
 4741 CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG
 4801 GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA
 4861 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT
 4921 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT
 4981 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC
 5041 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT
 5101 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT
 5161 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC
 5221 AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC
 5281 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG
 5341 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC
 5401 AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC
 5461 CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC
 5521 TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
 5581 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
 5641 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
 5701 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
 5761 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
 5821 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
 5881 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
 5941 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
 6001 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
 6061 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
 6121 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
 6181 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
 6241 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
 6301 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
 6361 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
 6421 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
 6481 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
 6541 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
 6601 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
 6661 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
 6721 CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA
 6781 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
 6841 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
 6901 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGcattaa
 6961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctCTTC CGCTTCCTCG
 7021 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
 7081 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
 7141 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
 7201 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
 7261 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
 7321 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
 7381 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
 7441 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
 7501 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
 7561 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
 7621 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA
 7681 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
 7741 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG
 7801 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
 7861 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
 7921 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
 7981 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
 8041 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA
 8101 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
 8161 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
 8221 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
 8281 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
 8341 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
 8401 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
 8461 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
 8521 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
 8581 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
 8641 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
 8701 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
 8761 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
 8821 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
 8881 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
 8941 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG
 9001 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT
 9061 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC
 9121 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCC
//